In contrast, normal subjects and patients with various pulmonary and extrapulmonary diseases other than pulmonary sarcoidosis had levels of CD8 antigen expression on lung lymphocytes similar to those on blood lymphocytes. Although the MFI of CD8 on lung lymphocytes was unexpectedly higher than that on blood lymphocytes in patients with pulmonary sarcoidosis, two-color immunofluorescence analysis […]

Since it has been shown that the down-regulation of the cell surface CD3/TCR complex is correlated with the inhibition of antigen recognition and related immune responses to antigens or mitogens,’* our observations suggest that the modulation of CD3/TCR is involved in the hyporesponsiveness of lung T cells. Such down-regulation would be of physiologic relevance to […]

However, several observations in the present study seem to be inconsistent with the concept delineated above. There was a positive correlation between the MFI of CD3 on lung lymphocytes and the proportion of BALF lymphocytes, when all study subjects are combined. In addition, a significant positive correlation was found between the MFI of CD3 on […]

Our study demonstrated for the first time that the CD3 antigen on lung lymphocytes is expressed in lower density than that expressed on blood lymphocytes. This phenomenon was observed in all normal subjects and all patients with various pulmonary and extrapulmonary disorders in the present study. Since CD3 is known to be noncovalently linked to […]

However, the CD8 antigen on lung lymphocytes was expressed in a significantly higher density than that expressed on blood lymphocytes in patients with pulmonary sarcoidosis (p<0.001). As can be seen in Figure 5, this was due to a decrease in the MFI of CD8 on blood lymphocytes in this disease, rather than to an increase […]

Because CD3 antigen is expressed on both CD4+ and CD8+ cells, it would be interesting to know in which subset of lung T cells CD3 molecules are more modulated. Two-color analysis in the limited number of subjects revealed that the amount of CD3 antigen on CD4+ lung lymphocytes was approximately equivalent to the amount present […]

Expression of CD3 Figure 1 shows a representative overlay histogram of lung and blood lymphocytes stained with anti-CD3 monoclonal antibody in a patient with pulmonary sarcoidosis. Although the proportion of CD3+ cells in lung lymphocytes was higher than that in blood, their MFI was apparently reduced as compared with that in blood. Similar results were […]