Category: Uterine

Uterine

The Follicle-Deplete Mouse Ovary Produces Androgen: DISCUSSION

4-Vinylcyclohexene is an industrial chemical used in the manufacture of rubber tires, polyesters, coatings, and plastics. Its diepoxide metabolite, 4-vinylcyclohexene diepoxide (VCD), has been extensively characterized for its ability to cause selective loss of small preantral (primordial and primary) follicles in ovaries of rats and mice after repeated dosing. Follicle…

Uterine

The Follicle-Deplete Mouse Ovary Produces Androgen: RESULTS

Characterization of the VCD-Treated Ovary In a detailed dose-response study, VCD treatment (80320 mg kg-1 day-1 effective dose) resulted in a significant reduction (P < 0.05) in both primordial and primary follicle pools relative to controls on Day 15 after the initiation of treatment (Fig. 1). Histological evaluation demonstrated that…

Uterine

The Follicle-Deplete Mouse Ovary Produces Androgen: MATERIALS AND METHODS

Animals Immature female B6C3Fj mice (21 days) were obtained from Harlan Laboratories (Indianapolis, IN) and The Jackson Laboratories (Bar Harbor, ME), housed in plastic cages, and maintained on 12L:12D cycles at 22 ± 2°C. Animals were allowed to acclimate to the animal facilities for 1 wk prior to initiation of…

Uterine

The Follicle-Deplete Mouse Ovary Produces Androgen: INTRODUCTION

At birth, the mammalian ovary contains a finite number of primordial follicles. The majority of these will never mature to ovulation. Rather, >99% undergo a process of degeneration, designated atresia. During the atretic process, granulosa cells and oocytes are eliminated via physiological cell death, apoptosis. In contrast, the theca interna…

Uterine

Development and Use of an Ovarian: DISCUSSION

Study of the sequential changes in UBF during normal ovine ovarian cycles is not possible during the anestrous period. Thus, to eliminate restrictions of studying seasonal photoreceptive breeders and to remove the confounding variability of cyclical changes in ovarian steroids, the chronically instrumented ovx sheep model was developed. The vast…

Uterine

Development and Use of an Ovarian: RESULTS

Uterine Blood Flow Changes During the Periovulatory Period Baseline levels of UBF were equal (P > 0.05) in the three ovary-intact groups, averaging 14.8 ± 1.7 ml/min. Regardless of which season (cycling or anestrous) this treatment regimen was used, a rise in UBF was observed in the 500- and 1000-IU-eCG…

Uterine

Development and Use of an Ovarian: MATERIAL AND METHODS

Animal Preparation Procedures for animal handling and protocols for experimental procedures were approved by the University of Wisconsin-Madison Research and Animal Care and Use Committees of both the Medical School and the College of Agriculture and Life Sciences. Nonpregnant, intact multip-arous ewes (50-65 kg) of mixed western breeds were exposed…