Ribonucleic Acid's archives

It is possible that the inhibitory action of estradiol may be overwhelmed by the stimulatory effect of rPL in the corpus luteum during midpregnancy. It is difficult to explain the physiological roles of the inhibitory effects of estradiol on SOD mRNA expression; however, estradiol may contribute to restricting the capacity of the corpus luteum to […]

This is, to our knowledge, the first report showing marked stimulatory effects of PRL and rPLs on both Cu,Zn-SOD and Mn-SOD mRNA expression in rat luteal cells. Pituitary PRL and placental PRL-like hormones such as rPLs are essential for the maintenance of luteal function in the rat. These hormones, in combination with estradiol, stimulate overall […]

Effects of PRL and rPL on Cu,Zn-SOD and Mn-SOD mRNA Expression in GG-CL Cells To examine whether PRL signaling to both SODs occurs through the long form of the PRL receptor, we used the SV-40 transformed luteal cell line (GG-CL) derived from the large luteal cell of the corpus luteum of pregnant rats, which was […]

PRL Regulation of Luteal Cu,Zn-SOD and Mn-SOD mRNA Expression In Vivo Since the corpus luteum is exposed to high levels of pituitary PRL in early pregnancy, and to even higher concentrations of placental PRL-related hormones later on in gestation, we examined the effect of PRL in vivo on luteal Cu,Zn-SOD and Mn-SOD mRNA expression. Rats […]

Isolation of Total RNA and RT-PCR Total RNA was isolated from corpora lutea by homog-enization in guanidinium thiocyanate and centrifugation through a cesium chloride cushion, whereas total RNA from the cultured cells was isolated by the guanidinium-isothiocyanate-phenol-chloroform extraction procedure. For mRNA analysis by RT-PCR, oligonucleotide primers for Cu,Zn-SOD (5′ -TTCGAGCAGAAGGCA-AGCGGTGAA-3′ and 5′ -AATCCCAATCACACCACAA-GCCAA-3′) and for […]

These colonies were selected and cultured in the growth medium containing 5% FBS until the cells were confluent. To identify successful stable transfection with the PRL receptor, cells were grown and passaged several times. PRL receptor mRNA expression in these cells was determined by reverse transcription-polymerase chain reaction (RT-PCR) using PRL receptor-specific primers as reported […]

After 72 h of incubation, the medium was changed, and the cells were treated for 12 h with various doses of PRL (NIDDK oPRL-20, 31 IU/mg), purified rat placental lactogen-I (rPL-I) or recombinant rPL-II kindly provided by Dr. M. Robertson (Department of Physiology, University of Manitoba, Winnipeg, MB, Healthcare Canada) at 37°C in the absence […]