Category: Ribonucleic Acid

Ribonucleic Acid

Hormonal Regulation of Copper-Zinc Superoxide Dismutase: MATERIALS AND METHODS(4)

Isolation of Total RNA and RT-PCR Total RNA was isolated from corpora lutea by homog-enization in guanidinium thiocyanate and centrifugation through a cesium chloride cushion, whereas total RNA from the cultured cells was isolated by the guanidinium-isothiocyanate-phenol-chloroform extraction procedure. For mRNA analysis by RT-PCR, oligonucleotide primers for Cu,Zn-SOD (5′ -TTCGAGCAGAAGGCA-AGCGGTGAA-3′…