Category: Pulmonary function

Pulmonary function

Techniques in Human Airway Inflammation: Discussion

There were no differences in the quality of immu-nostaining in the sections of the biopsy specimens taken with the three types of biopsy forceps. However, the possibilities to count reproducible cell numbers were best in biopsy specimens taken with the FB-21C, being largely due to the presence of more intact…

Pulmonary function

Techniques in Human Airway Inflammation: Materials and Methods

Mucosal biopsy specimens were immediately transported to the laboratory on a slide in a humidified, ice-cooled container, and subsequently embedded (in TissueTek; Sakura Finetek USA; Torrance, Calif) and snap-frozen by immersing in precooled isopentane at —80°C. Sections were cut at 4 μm and stored at —80°C until use. To evaluate…

Pulmonary function

Techniques in Human Airway Inflammation

Quantity and Morphology of Bronchial Biopsy Specimens Taken by Forceps of Three Sizes Fiberoptic bronchoscopy is at present a routine diagnostic procedure in pulmonary diseases. In recent years, this procedure has been introduced in the research of pathophysiologic mechanisms of bronchial asthma. It appears to be a safe procedure, even…