Category: Ovarian

Ovarian

Sperm-Induced Modification of the Oviductal Gene: DISCUSSION

Two previous in vitro studies investigated the possibility that spermatozoa can induce de novo protein synthesis. Novel protein synthesis was apparently detected in cultured oviduct cells to which spermatozoa were added. However, the proteins could not be defined. The results of the present study strongly support the concept that spermatozoa…

Ovarian

Sperm-Induced Modification of the Oviductal Gene: RESULTS

Expression levels of 11 263 transcripts (93% of total transcripts present on the oligonucleotide array) consistently increased, decreased, or remained unchanged (in four or more comparisons out of six) when the oviductal transcriptome of mice in estrus was compared with that of mice 6 h after mating. Expression levels of…

Ovarian

Sperm-Induced Modification of the Oviductal Gene: MATERIALS AND METHODS

Oviduct Collection and RNA Isolation Outbred TO female mice (Harlan, Oxon, U.K.) were used in these experiments. All procedures relating to the care and use of animals were performed according to United Kingdom Home Office regulations, Animals (Scientific Procedures) Act 1986. Oviducts were collected after the animals were killed from…

Ovarian

Sperm-Induced Modification of the Oviductal Gene: INTRODUCTION

The mammalian oviduct is the venue of important events leading to the establishment of pregnancy. These events include final maturation and transport of the female and male gametes, fertilization, early cleavage-stage embryonic development, and transport of the embryo to the uterus. In mammals, the physiological interaction between gametes, embryos, and…

Ovarian

Molecular Cloning and Ovarian Expression Profiles of Thrombospondin: RESULTS

Microsequencing of CR Proteins To examine a sequence similarity of CR proteins that displayed immunological similarity in our previous study, N-terminal sequences of the 150- and 130-kDa proteins were analyzed by microsequencing. The sequences were identical in the two proteins: SVTTAYHLYSYLCEGQ. The 150-kDa protein was treated with lysyl endopeptidase, and…

Ovarian

Molecular Cloning and Ovarian Expression Profiles of Thrombospondin: MATERIALS AND METHODS

Source of Shrimp and Classification of Ovarian Developmental Stages The kuruma prawn (M. japonicus) was purchased from a commercial source in Mie, Japan, or provided from the Momoshima Branch of the National Farming Center, Hiroshima, Japan. Ovarian stages were determined from histological observation of paraffin sections as follows: immature stage—yolkaccumulationhasnotyetoccurred,addthereforethe…