Category: Hormone

Hormone

Mouse Follicle-Stimulating Hormone: DISCUSSION

The central role of FSH in development and function of the mammalian reproductive system means that measurement of FSH is vital for research in reproductive physiology, just as it has been critical in clinical reproductive medicine. In females, FSH is essential for ovarian development, follicle recruitment and growth, as well…

Hormone

Mouse Follicle-Stimulating Hormone: RESULTS

Assay Characteristics The IFMA for mFSH was 34-fold more sensitive than RIA. The IFMA could detect 5 pg/well (10% of dynamic range) using a 25-^l serum sample per well as compared to conventional RIA using a 100-^l serum sample per tube with a sensitivity of 170 ± 11 pg/tube at…

Hormone

Mouse Follicle-Stimulating Hormone: MATERIALS AND METHODS

mFSH and mLH RIA Mouse FSH was measured by double-antibody RIA using reagents from National Institute of Diabetes and Digestive and Kidney Diseases as described originally and updated by the National Hormone and Peptide Program. Briefly, 100 |xl of serum or mouse standard solution (1-125 ng/ml, AFP5308D) were incubated with…

Hormone

Mouse Follicle-Stimulating Hormone: INTRODUCTION

A heterodimeric pituitary glycoprotein hormone, FSH plays a major role in mammalian reproduction. It is a heterodimer comprising an a subunit identical to that of LH, hCG, and thyrotropin combined with a p subunit homologous with those of the other three members of the glycoprotein hormone family. The p subunit…