Category: Cell

Cell

An Overview of Cell Renewal in the Testis: DISCUSSION

High cell proliferation in the gilthead seabream during spermatogenesis results in an increased GSI and larger gonad area, whereas fish growth ceases in favor of testis development. At the end of spermatogenesis, the tubules and the efferent duct possess wide lumina full of free spermatozoa. The shedding of spermatozoa leads…

Cell

An Overview of Cell Renewal in the Testis: RESULTS

Reproductive Cycle of Gilthead Seabream Males The reproductive cycle of the gilthead seabream, a protandrous hermaphrodite teleost, can be divided into four stages: spermatogenesis, an asynchronous process that lasts for several months (from September to February), spawning (March), postspawning (from April to May), and resting (from June to September). The…

Cell

An Overview of Cell Renewal in the Testis: MATERIAL AND METHODS

Fish Healthy specimens of sexually mature male gilthead seabream Sparus aurata (Sparidae, Perciform, Teleostei), with a body weight (BW) of 100 g, were obtained in November 2002, from Predomar, S.L. (Carboneras, Spain). The fish were kept at the Spanish Oceanographic Institute (Ma-zarron, Murcia), in 14 m3 running-seawater aquaria (dissolved oxygen…

Cell

An Overview of Cell Renewal in the Testis: INTRODUCTION

Spermatogenesis, the formation of sperm, is a complex process in which spermatogonia divide and differentiate into spermatozoa. In fish, spermatogenesis appears to proceed in a similar fashion to that observed in other vertebrates, although with some important differences. In mammals, the germinal epithelium is constant throughout the year or it…

Cell

Cell Donor Influences Success of Producing Cattle: DISCUSSION

More than 900 good-quality blastocysts were generated in the above three experiments. About two thirds were transferred to embryo recipients. Because calving rates were low, it was not possible to make a rigorous assessment of the impact of in vitro treatments on overall somatic cell nuclear transfer success. However, some…

Cell

Cell Donor Influences Success of Producing Cattle: RESULTS

Experiment 1. Influence of Culture Media and Fibroblast Tissue Source Blastocyst development was evaluated in a 2 X 3 X 2 factorial experimental design. The ability of fibroblasts isolated from hip muscle or lung tissue of four fetal fibroblast sources cultured in MEM or DMEM were compared for their ability…